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Experiment 14: Reaction of Crystal Violet with NaOH
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How do you measure the rate of a crystal violet reaction?
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The kinetics of this reaction can be monitored with a spectrophotometer by observing the decrease in absorbance of crystal violet with time. The rate law in general form is: rate of disappearance of CV = rate of appearance of CVOH = k [CV]x [OH-]y (1)
What is the rate law of crystal violet?
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Crystal violet is a common, beautiful purple dye. In strongly basic solutions, the bright color of the dye slowly fades and the solution becomes colorless. The kinetics of this “fading” reaction can be analyzed by measuring the color intensity or “absorbance” of the solution versus time to determine the rate law.
What is the rate law for the crystal violet reaction with respect to concentration of crystal violet? The rate law for reaction (1) is of the form: rate = k [CV+]m[OH–]n, where k is the rate constant for the reaction m is the order with respect to crystal violet (CV+) n is the order with respect to the hydroxide ion.
What is the concentration of CV for Crystal violet?
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CAUTION! Crystal violet will stain skin and clothing! Record the concentration of CV (it should be around 2 x 10 -5 M). Prepare the spectrometer and computer to collect absorbance data as described on the Ocean Optics page (the instrument should be already connected to a computer and warming up when you arrive).
What is crystal violet used for in microbiology?
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Crystal violet is also used as a biological stain. The process of Gram staining to classify bacteria exposes bacteria to crystal violet. Depending the type of bacteria, the stain containing crystal violet will cause the bacteria to turn one color or another. Crystal violet can be used is DNA gel electrophoresis and fingerprinting.
References:
LAB #7.1: KINETICS OF CRYSTAL VIOLET – CHEMDUNN
Experiment Three: The Crystal Violet Experience | PDF
Kinetics of Crystal Violet Bleaching | Chem Lab
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